INDICATORS OF OXIDATIVE STRESS IN RATS WITH PURULENT NECROTIC SKIN WOUNDS
DOI: https://doi.org/10.17721/1728.2748.2025.102.41-47
Keywords:
Purulent necrotic wounds, lipid peroxidation, composition based on proteolytic enzymes.Abstract
Background. Purulent necrotic skin lesions are a complex pathophysiological problem accompanied by intense inflammation, bacterial contamination, and increased oxidative stress, which destabilizes regeneration processes. One of the important mechanisms of pathogenesis is the activation of lipid peroxidation (LPO), which causes damage to cellular structures and inhibits healing. In this context, the search for agents capable of modulating prooxidant-antioxidant homeostasis is relevant. A promising direction is the use of compositions based on proteolytic enzymes of natural origin, in particular from aquatic organisms. The aim of the study was to evaluate lipid peroxidation in rats with purulent necrotic wounds when using a composition based on enzymes from the aquatic organisms sea urchin Sterechinus neumayeri and starfish Odontaster validus. Translated with DeepL.com (free version).
Methods. The study used 88 white nonlinear rats, which were modeled with purulent necrotic wounds up to 400 mm². The content of diene conjugates, cipher bases, TBA-active products and hydrogen peroxide in skin homogenate and blood serum was determined on days 3, 6, 9, 14 of the experiment and after complete skin reepithelialization using a composition based on proteolytic enzymes purified from aquatic organisms of the Antarctic region (sea urchin Sterechinus neumayeri and starfish Odontaster validus).
Results An increase in the content of lipid peroxidation products in the blood serum of rats with purulent necrotic wounds was found: on average, DC 2-fold, SHO 2.5-fold, TBA-active products 2.5-fold. In the skin homogenate of rats, the following were increased on average: DC - 2.4 times, SHO - 1.9 times, TBA-active products - 2.7 times, During the treatment of wounds with a composition based on enzymes of marine hydrobionts, a decrease in indicators to control ones was observed, starting from day 14 of the experiment.
An increase in the concentration of hydrogen peroxide in the blood serum of rats with purulent necrotic skin wounds was detected throughout the experiment. The use of a composition based on proteolytic enzymes contributed to a 1.3-fold decrease in the index on average.
Conclusions. Studies have shown a significant increase in the serum and skin homogenate of rats with purulent necrotic wounds in all periods of wound healing. In the group of animals treated with a composition based on proteolytic enzymes once a day, the studied parameters gradually returned to control values.
References
Avila-Rodríguez, M., Meléndez-Martínez, D., Licona-Cassani, C., Aguilar-Yañez, J., Benavides, J., & Sánchez, M. (2020). Practical context of enzymatic treatment for wound healing: A secreted protease approach (Review). Biomedical Reports, 13(1), 3–14. https://doi.org/10.3892/br.2020.1356
Bakiu, R., Piva, E., Pacchini, S., & Santovito, G. (2024). Antioxidant Systems in Extremophile Marine Fish Species. Journal of Marine Science and Engineering, 12(8), 1280. https://doi.org/10.3390/jmse12081280
Beeton, C., & Jackson, J. (2013). Proteases and delayed wound healing. Expert Opinion on Therapeutic Targets, 17(10), 1239–1250. https://doi.org/10.1517/14728222.2013.827661
Catalá, A. (2009). Lipid peroxidation of membrane phospholipids generates hydroxy-alkenals and oxidized phospholipids active in physiological and/or pathological conditions. Chemistry and Physics of Lipids, 157(1), 1–11. https://doi.org/10.1016/j.chemphyslip.2008.09.004
Cordiano, R., Gioacchino, M., Mangifesta, R., Panzera, C., Gangemi, S., & Minciullo, P. (2023). Malondialdehyde as a potential oxidative stress marker for allergy-oriented diseases: An update. Molecules, 28(16), 5979. https://doi.org/10.3390/molecules28165979
Council of Europe. (1986). European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. Strasbourg: Council of Europe. https://rm.coe.int/168007a67b
Dvorshchenko, K., Kovaleva, V., & Kharchenko, O. (2007). Lipid peroxidation in rat hepatocytes under conditions of gastric ulcer. Visnyk of Taras Shevchenko National University of Kyiv. Series: Biology, 49(1), 54–55 [in Ukrainian].
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 38(12), 1103–1111.
Feng, J., Wang, J., Wang, Y., Huang, X., Shao, T., Deng, X., Cao, Y., Zhou, M., & Zhao, C. (2022). Oxidative stress and lipid peroxidation: Prospective associations between ferroptosis and delayed wound healing in diabetic ulcers. Frontiers in Cell and Developmental Biology, 10, 898657. https://doi.org/10.3389/fcell.2022.898657
Han, G., & Ceilley, R. (2017). Chronic wound healing: a review of current management and treatments. Advances in therapy, 34(3), 599–610.
Jarc, E., & Petan, T. (2019). Lipid Droplets and the Management of Cellular Stress. Yale J Biol Med, 92(3), 435-452.
Jomova, K., Raptova, R., Alomar, S., Alwasel, S., Nepovimova, E., Kuca, K., & Valko, M. (2023). Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Archives of toxicology, 97(10), 2499–2574.
Katikaneni, A., Jelcic, M., Gerlach, G., Ma, Y., Overholtzer, M., & Niethammer, P. (2020). Lipid peroxidation regulates long-range wound detection through 5-lipoxygenase in zebrafish. Nature cell biology, 22(9), 1049–1055.
Khorsandi, K., Hosseinzadeh, R., Esfahani, H., Zandsalimi, K., Shahidi, F., & Abrahamse, H. (2022). Accelerating skin regeneration and wound healing by controlled ROS from photodynamic treatment. Inflammation and Regeneration, 42, 40. https://doi.org/10.1186/s41232-022-00226-6
Martinengo, L., Olsson, M., Bajpai, R., Soljak, M., Upton, Z., Schmidtchen, A., Car, J., & Järbrink, K. (2019). Prevalence of chronic wounds in the general population: systematic review and meta-analysis of observational studies. Annals of epidemiology, 29, 8–15.
Monroe, T., Hertzel, A., Dickey, D., Hagen, T., Santibanez, S., Berdaweel, I., Halley, C., Puchalska, P., Anderson, E., Camell, C., Robbins, P., & Bernlohr, D. (2025). Lipid peroxidation products induce carbonyl stress, mitochondrial dysfunction, and cellular senescence in human and murine cells. Aging Cell, 24(1), e14367. https://doi.org/10.1111/acel.14367.
Rajkumar, J., Chandan, N., Lio, P., & Shi, V. (2023). The skin barrier and moisturization: function, disruption, and mechanisms of repair. Skin pharmacology and physiology, 36(4), 174–185.
Sen, C., & Roy, S. (2008). Redox signals and wound healing. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research, 1780(11), 1348–1361.
Sharma, P., Jha, A., Dubey, R., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany [online], 2012. https://doi.org/10.1155/2012/217037
Tsikas, D. (2017). Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem, 524, 13–30.
Verkhovna Rada of Ukraine. (2006). On the Protection of Animals from Cruelty. Law of Ukraine No. 3447–IV. Bulletin of the Verkhovna Rada of Ukraine, 27, 230 [in Ukrainian]. https://zakon.rada.gov.ua/laws/show/3447-15
Yang, J., Luo, J., Tian, X., Zhao, Y., Li, Y., & Wu, X. (2024). Progress in understanding oxidative stress, aging, and aging-related diseases. Antioxidants, 13(4), 394. https://doi.org/10.3390/antiox13040394
