CONTENT OF MIDDLE MOLECULAR PEPTIDES IN THE TISSUES OF RATS WITH ALKALI BURNS OF THE ESOPHAGUS
DOI: https://doi.org/10.17721/1728.2748.2025.103.28-34
Keywords:
alkaline burn of the esophagus, immature rats, middle molecular peptidesAbstract
Background. Corrosive substance ingestion remains a significant public health issue which leads to burns of the upper parts of the gastrointestinal tract, often accompanied by burns of the respiratory tract or pharynx for children. Just under 6 % of acute cases result in death due to multi-organ failure. Survivors of the acute phase frequently experience long-term complications due to scarring, which may affect the oesophagus and stomach. The aim of the study was to determine the level of middle molecular peptides as a parameter of endogenous intoxication in rat's organs homogenates of immature rats in accordance with the model of the 1st and 2nd degree an alkali esophageal burn.
Methods. The model of an alkali esophageal burn was reproduced using female rats (1 month); the animals were kept on a standard vivarium diet. The alkali esophageal burn (1st degree) was stimulated using 10 % NaOH and 2nd degree ‒ 20 % NaOH, respectively. We have used the Bradford method to estimate the concentration of protein. The level of middle molecular peptides was determined at wavelengths λ = 210, 238 and 254 nm.
Results. We have demonstrated the endogenous intoxication syndrome modeling an alkali esophageal burn in immature rats. In the study of middle molecular peptides upon the burns of the 1st degree we observed the increase of their concentration on the 14 day of the experiment in the liver homogenate; in the kidney – on the 7th and 21st day; in the muscle homogenate –throughout the entire research period; in the brain – on the 21st day of the experiment. As for the burns of the 2nd degree we obtained the increase of middle mass peptides concentration in the liver, kidney and muscle homogenates preferentially on the 7st day of the experiment; in the brain – on the 21st day and in the esophageal mucosa – on the 1st day of the experiment.
Conclusions. The results obtained indicate that numerous pathological disorders in the immature rat's organism after an alkali esophageal burn. The increased levels of toxins of various nature are associated with a high risk of septic complications after burns. Therefore, the level of middle molecular mass peptides is a convenient clinical indicator characterizing the dynamics of the development of pathological processes.
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