THE STATE OF ANTIOXIDANT SYSTEM IN RATS WITH PURULENT-NECROTIC SKIN WOUNDS
DOI: https://doi.org/10.17721/1728.2748.2025.103.66-74
Keywords:
Purulent necrotic wounds, SOD, catlase, glutathione, composition based on proteolytic enzymes.Abstract
Background. Purulent necrotic skin lesions pose a significant medical problem, as they are accompanied by a complex disruption of physiological processes, which significantly complicates healing. One of the key pathogenetic links in the development of these conditions is oxidative stress. It is important to study the state of the antioxidant system, as it is the body's natural defense mechanism that counteracts the harmful effects of free radicals and lipid peroxidation products (LPO). In severe injuries such as purulent necrotic wounds, the body's own antioxidant reserves are often insufficient to effectively control oxidative stress. Therefore, it is important to search for agents capable of modulating prooxidant-antioxidant homeostasis. A promising direction is the use of compositions based on proteolytic enzymes of natural origin, in particular from hydrobionts. The aim of the study was to evaluate the state of the antioxidant system in the blood serum and skin homogenate of rats with purulent-necrotic wounds during natural healing and with the use of a composition based on enzymes purified from aquatic organisms – sea urchin Sterechinus neumayeri and starfish Odontaster validus.
Methods. The study was conducted in 88 white nonlinear rats, which were modeled with purulent necrotic wounds up to 400 mm². The activity of the enzymes superoxide dismutase (SOD), catalase, glutathione peroxidase and the content of oxidized and reduced glutathione in the skin homogenate and blood serum were determined on days 3, 6, 9, 14 days of the experiment and after complete reepithelialization of the skin using a composition based on proteolytic enzymes purified from the Antarctic region aquatic organisms (sea urchin Sterechinus neumayeri and starfish Odontaster validus)
Results In purulent necrotic skin wounds, changes in the activity of antioxidant defense enzymes were shown: a decrease in superoxide dismutase, an increase in catalase and glutathione peroxidase activity against a decrease in GSH concentration, both in blood serum and in skin homogenate. The use of the enzyme-based composition for the treatment of wounds restored the indicators to the control level starting from day 9 of the experiment.
Conclusions. The application of a composition based on enzymes purified from hydrobionts – sea urchin Sterechinus neumayeri and starfish Odontaster validus – to purulent-necrotic wounds contributed to the restoration of antioxidant protection, which, in turn, contributes to better resistance to free radicals and faster skin regeneration.
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