EVOLUTIONARY RELATIONSHIP OF UKRAINIAN ISOLATE OF ODONTOGLOSSUM RINGSPOT VIRUS

Abstract

Odontoglossum ringspot virus (ORSV) is one of the most dangerous viruses of decorative orchids. Infection caused by this virus can reduce the phenotypic differences of orchids in collections, and as a result lead to their total exhaustion. In this study, the possible origin of the Ukrainian isolate of ORSV from the collection of O.V. Fomin Botanical Garden of the Taras Shevchenko National University of Kyiv was investigated. The properties of nucleotide sequences of the RNA-depended RNA-polymerase (RdRp) and coat protein gene of ORSV were examined. RNAs of ORSV isolated from leaves of Cymbidium hybridum from O.V. Fomin Botanical Garden’s collection were amplified through RT-PCR and sequenced. Obtained sequences were compared at the nucleotide level with ORSV isolates accessible within the GenBank. Phylogenetic examination appeared that the studied ORSV isolate may have a common root with a few South Korean isolates.