THE EFFECT OF ALCOHOL ON BLOOD LIPID COMPOSITION AND LIPOPEROXIDATION PROCESSESIN BRAIN TISSUE OF RATS WITH DIFFERENT ALCOHOL MOTIVATIONAND DIFFERENT LEARNING ABILITY
DOI: 10.17721/1728.2748.2024.96.9-14
Keywords:
Lipid metabolism, lipid peroxidation, alcohol motivation, learningAbstract
B a c k g r o u n d . Alcohol, as a factor of negative influence on the organism, induces metabolic changes and may affect cognitive task performance, asnervous tissue is particularly sensitive to ethanol exposure. This sensitivity is manifested through oxidative processes in the brain and alterations in lipid metabolism. The study investigated the sensitivity of oxidative processes in brain tissue and changes in the blood lipid spectrum under the influence of ethanol with different combinations of alcoholization and trainingin rats.
M e t h o d s . Male rats aged three to five months were used in the study. Learning ability was assessed in the radialmaze. The impact of ethanol on metabolic processes wase valuated by determining the lipid levels in blood plasma usingthin-layer chromatography. The effect of ethanol and training on brain tissues was examined by determining the amount of malondialdehyde in rat brain tissues spectrophotometrically using the 2-thiobarbituric acidtest.
R e s u l t s . Unequali poperoxidative reactivity of rat brain tissues with different alcohol motivations was demonstrated under various combinations of ethanol and training. Rats showing no preference for alcohol exhibited higher lipoperoxidative reactivity, especially those that performed poorly in learning and consumed ethanol after training. Animals that learned well had a lowerlevel of peroxide oxidation after alcohol consumption. Rats preferring alcohol demonstrated the lowest degree of peroxideoxidation in brain tissues.
C o n c l u s i o n s . Chronic alcoholism led to alterations in the lipid concentration in the rats' blood, indicating a moderate destabilizing effect of ethanol on lipid metabolism under the conditions of our experiment. High levels of lipid peroxidation in rat brain tissues are characteristic of rats with low alcohol motivation, while training rat spriorto the onset of alcoholismmayreduce the level of lipidperoxidation.
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