EXPRESSION GENES OF CARTILAGE STRUCTURAL PROTEINS OF PATIENTS WITH OSTEOARTHRITIS AFTER SARS-COV2 INFECTION
DOI: 10.17721/1728.2748.2024.97.5-9
Keywords:
SARS-CoV-2, osteoarthritis, synovial fluid, inflammation, COL2A1, СOMP gene expressionAbstract
B a c k g r o u n d . The emergence of severe acute respiratory syndrome caused by coronavirus 2 (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) has caused significant global morbidity and mortality in various countries of the world, including Ukraine. The severity of the course of the 2019 coronavirus disease (Coronavirus disease 2019, COVID-19) and the risk of complications may depend on the presence of chronic diseases. Osteoarthritis is one of the most common chronic diseases of the musculoskeletal system. Inflammatory and degenerative processes in osteoarthritis are accompanied by a violation of the structural integrity of the joint. Components of the extracellular matrix, such as proteoglycans and collagens, together with chondrocytes, provide the mechanical and biological integrity of cartilage. Among the structural proteins of cartilage, collagen type II alpha 1 chain (COL2A1) and cartilage oligomeric matrix protein (COMP) will play an important role.
M e t h o d s . The aim of the work was to determine the expression of the COL2A1 and COMP genes in synovial fluid cells of patients with osteoarthritis after SARS-CoV2 infection. All study participants were divided into two groups. The first group (n = 22) is patients with osteoarthritis of the knee joints II-III degree. The second group (n = 14) is patients with osteoarthritis of the knee joints II-III degree, who suffered mild and moderate form of COVID-19 6–9 months ago. The expression level of COL2A1 and COMP genes was determined in nuclear cells of the synovial fluid, which was evaluated using the method of quantitative polymerase chain reaction with detection of results in real time. Processing of research results was carried out using generally accepted methods of variational statistics.
R e s u l t s . In patients with osteoarthritis of the knee joints after suffering from the disease of COVID-19, the expression of COL2A1 and СOMP genes in synovial fluid cells is reduced compared to patients with osteoarthritis who did not suffer from coronavirus infection. The obtained data indicate the intensification of destructive processes in the cartilage tissue of the joints of patients with osteoarthritis after infection with SARS-CoV-2.
C o n c l u s i o n s . The results obtained by us indicate that patients with osteoarthritis may develop a more severe course of synovial inflammation after infection with COVID-19.
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