DETECTION OF CUCUMBER MOSAIC VIRUS IN NOVEL HOST PLANTS IN UKRAINE
DOI: https://doi.org/10.17721/1728.2748.2025.103.10-16
Keywords:
CMV, Bromoviridae, host range, enzyme-linked immunosorbent assay, identificationAbstract
Background. Cucumber mosaic virus (CMV; species Cucumovirus CMV, genus Cucumovirus, family Bromoviridae) has the widest host range among plant viruses, infecting over 1,000 species across diverse genera and families. Various CMV strains and isolates are constantly evolving, adapting to new hosts, and may pose significant threats to the cultivation of economically important crops.
Methods. This study aimed to detect CMV in atypical host plants in Ukraine.Double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) was employed to identify CMV and to exclude mixed infections with other viruses, including alfalfa mosaic virus (AMV), arabis mosaic virus (ArMV), impatiens necrotic spot virus (INSV), potato virus X (PVX), potato virus Y (PVY), soybean mosaic virus (SMV), tobacco mosaic virus (TMV), tobacco rattle virus (TRV), tobacco ringspot virus (TRSV), tomato mosaic virus (ToMV), tomato spotted wilt virus (TSWV), turnip mosaic virus (TuMV), and turnip yellow mosaic virus (TYMV), using commercial ELISA kits (Loewe Biochemica GmbH, Germany).
Results. Screening of agroecosystems and ornamental plantings in several regions of Ukraine revealed CMV-infected plants. CMV monoinfections were detected in Hosta longissima (Asparagaceae), Echinacea purpurea, Erigeron annuus, and Heliopsis helianthoides (Asteraceae), Alliaria petiolata (Brassicaceae), Silene latifolia (Caryophyllaceae), and Euphorbia epithymoides (Euphorbiaceae).
Conclusions. These findings demonstrate the circulation of CMV in different regions of Ukraine across host plants from diverse families, including Asparagaceae, Asteraceae, Brassicaceae, Caryophyllaceae, and Euphorbiaceae. Notably, CMV was detected for the first time in E. annuus, H. helianthoides, and E. epithymoides, expanding the known host range of this economically significant virus in the Bromoviridae family.
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