ВИЯВЛЕННЯ CUCUMBER MOSAIC VIRUS НА НОВИХ РОСЛИНАХ-ХАЗЯЯХ В УКРАЇНІ
DOI: https://doi.org/10.17721/1728.2748.2025.103.10-16
Ключові слова:
CMV, Bromoviridae, спектр хазяїв, ідентифікація,, імуноферментний аналіз.Анотація
Вступ. Сucumber mosaic virus (Cucumovirus, Bromoviridae) має найширший спектр рослин-хазяїв серед усіх вірусів, він уражує понад 1000 видів рослин із різних родів і родин. Існують різні штами та ізоляти зазначеного збудника, які постійно еволюціонують, адаптуються до нових хазяїв і можуть становити потенційну небезпеку для виробництва цінних культур. Метою цього дослідження було виявити сucumber mosaic virus на нетипових рослинах-хазяях в Україні.
Методи. Імуноферментний аналіз у модифікації "сендвіч" (DAS-ELISA) використовували для ідентифікації cucumber mosaic virus (CMV) і для спростування змішаної інфекції з іншими вірусами, зокрема alfalfa mosaic virus (AMV), arabis mosaic virus (ArMV), impatiens necrotic spot virus (INSV), potato virus X (PVX), potato virus Y (PVY), soybean mosaic virus (SMV), tobacco mosaic virus (TMV), tobacco rattle virus (TRV), tobacco ringspot virus (TRSV), tomato mosaic virus (ToMV), tomato spotted wilt virus (TSWV), turnip mosaic virus (TuMV) та turnip yellow mosaic virus (TYMV) із застосуванням комерційних тест-систем (Loewe Biochemica GmbH, Germany).
Результати. Проведено скринінг агробіоценозів і зелених насаджень у населених пунктах різних областей України на наявність рослин, уражених cucumber mosaic virus. Виявлено моноінфекцію CMV у рослинах хости довгої (Hosta longissima) родини Asparagaceae; ехінацеї пурпурної (Echinacea purpurea), злинки однорічної (Erigeron annuus) і соняшничка садового (Heliopsis helianthoides) родини Asteraceae; часникової трави (Alliaria petiolata) родини Brassicaceae; смілки широколистої (Silene latifolia) родини Caryophyllaceae; молочаю різнобарвного Euphorbia epithymoides родини Euphorbiaceae.
Висновки. Показано циркуляцію CMV у різних регіонах України на рослинах-хазяях із різних родин, зокрема Asparagaceae, Asteraceae, Brassicaceae, Caryophyllaceae та Euphorbiaceae. Уперше виявлено зазначеного збудника на нових хазяях: E. annuus, H. helianthoides і E. epithymoides. Отримані дані розширюють знання про коло рослин-хазяїв найбільш небезпечного вірусу родини Bromoviridae.
Посилання
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