FUNCTIONAL CHARACTERISTICS OF PERITONEAL PHAGOCYTES IN RATS WITH 6-OHDA- AND LPS-INDUCED PARKINSON'S DISEASE
DOI 10.17721/1728.2748.2022.88.41-46
Keywords:
Parkinson's disease, peritoneal macrophages, MALT, reactive oxygens pecies, 6-OHDA, LPSAbstract
Peritoneal macrophages (PMs) refer to omentum-associated lymphoid tissue (OALT), which shares many features with mucosal-associated lymphoid tissue (MALT). However, the biological characteristics of this tissue have not yet been fully elucidated. The results of a significant amount of experimental data convincingly illustrate the integrative role of OALT and PM in its composition, in systemic inflammation, which is an important component of the pathophysiology of numerous diseases of inflammatory etiology. Such pathologies include Parkinson's disease (PD), a chronic systemic inflammatory disease, a deep understanding of the pathophysiology of which is necessary for the development of methods for its early diagnosis, prevention, and treatment. In this work, we compared the metabolic and phenotypic parameters of PM in rats with different models of PD: induced by intracerebral administration of 6-hydroxydopamine (6-OHDA) and bacterial lipopolysaccharide (LPS). The development of PD was ascertained by the results of behavioral tests. The phenotypic and functional properties of PM were characterized by their phagocytic activity, oxidative metabolism, and the level of expression of markers of proand anti-inflammatory polarized activation (CD80/86 and CD206, respectively), which were determined by flow cytometry. The development of PD in animals with both models was accompanied by behavioral disorders, which were more pronounced in animals with the LPS-induced model. The phenotypic and functional characteristics of the PM of animals with 6-OHDA- induced PD indicate their functional exhaustion as a result of a prolonged systemic inflammatory process. The functional state of the PM in rats with LPS-induced disease indicates their involvement in the spontane ous resolution of systemic inflammation.
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